Oocyte viability
Web19 de abr. de 2024 · Figure 1. Oocyte and embryo viability evaluation by methyl tetrazolium (MTT) staining in control and vitrified groups: (A) representative image for oocyte viability evaluation criteria (continuous arrows show purple living cells, and dotted arrows show colorless dead cells); 10×, scale bar = 50 μm; (B) percentage of oocyte … Web4 de jul. de 2024 · In this chapter, indications about the equipment, procedure, and timing to perform oocyte denudation that should be adopted in order to minimize any potential damage to oocyte viability will be reviewed. Keywords. Cumulus oophorus; Oocyte denudation; Oocyte morphological assessment; Gene expression analysis; Microfluidic …
Oocyte viability
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WebNational Center for Biotechnology Information WebHow does vitrification affect oocyte viability? Summary answer: Vitrification does not affect oocyte viability in oocyte donation cycles. What is known already: Oocyte vitrification …
Web20 de abr. de 2024 · Oocytes develop to maturity from within a follicle. These follicles are found in the outside layer of the ovaries. During each reproductive cycle, several follicles … Web7 de jul. de 2024 · Here, we describe a reliable protocol to assess coral oocyte viability with low per-test cost, simplicity for routine use, rapid execution and to obtain efficient …
Web1 de out. de 2024 · The evaluation of oocyte viability in the laboratory is limited to the morphological assessment by naked eyes, but the realization that most … Web16 de out. de 2024 · Atp5e and Atp5o were enriched in the most significant gene ontology term “mitochondrial membrane part”; thus, these genes may be promising candidate biomarkers for oocyte viability after IVM.
Web17 de nov. de 2024 · This study aimed to investigate the effects of different concentrations of N-acetylcysteine (NAC) on the growth, antrum formation, viability, and ultrastructure of bovine secondary follicles cultured in vitro for 18 days. To this end, the follicles were cultured in TCM-199+ medium alone or supplemented with 1.0, 5.0, or 25.0 mM NAC. Follicular …
WebCat oocyte cryopreservation is still considered to be an experimental technique (Luvoni 2006). The aim of this study was to evaluate the effect of different vitrification protocols applied to feline oocytes at different maturational stages on the preservation of oocyte viability and integrity of subcellular structures. grand rapids golf clubWeb21 de jan. de 2013 · Mouse.Oocytes that have had their mitochondria damaged or have not converted from glycolytic to aerobic respiration have a lower likelihood of development after fertilization [29, 86].Early studies demonstrated the feasibility of injection of mitochondria into an oocyte to increase intracellular ATP concentrations [].Mitochondrial injections … chinese new year edmontonWebTaken together, these data indicate that canine oocytes exhibited sensitivity to lesser temperatures and maintained greater rates of viability during transport at 37 … chinese new year early years activityWeb17 de nov. de 2024 · This study aimed to investigate the effects of different concentrations of N-acetylcysteine (NAC) on the growth, antrum formation, viability, and ultrastructure of … grand rapids gold ticketsWebOocyte viability assessed using histological analysis showed that vitrification solution 2.0 M Me 2 SO + 2.5 M etilenoglycol +0.5 M sucrose (VS3; 66.43 ± 4.68%) and 1.5 M … chinese new year ecards freeWebthe most widely used technique for oocyte cryopreservation in daily practice as it achieves outcomes similar to those obtained with fresh oocytes (Almodin et al., 2010; Cobo et al., 2010; Rienzi ... chinese new year dumplings nameWeb21 de jun. de 2024 · After cryobank storage for 14 days, experimental samples were thawed and oocyte viability was analyzed by oocyte morphology assessment and fluorescent microscopy. Two different kits were used to stain oocytes, one specific for necrosis/apoptosis (Propidium iodide/Alexa Fluor 488 Annexin) and the other specific for … grand rapids gold score