Nettet22. sep. 2024 · By using multimodal intersection analysis (MIA) to integrate scRNA-seq and microarray-based spatial transcriptomics (ST) methods, we presented the TME … http://ch.whu.edu.cn/en/article/doi/10.13203/j.whugig20240278
Integrating microarray-based spatial transcriptomics and single-cell
Nettet3. jun. 2024 · Background: Research on spatiotemporal gene landscape can provide insights into the spatial characteristics of human kidney development and facilitate kidney organoid cultivation. Here, we profiled the spatiotemporal gene programs of the human embryonic kidneys at 9 and 18 post-conception weeks (PCW) by integrating the … Nettet1. feb. 2024 · Integrating microarray-based spatial transcriptomics and single-cell RNA-seq reveals tissue architecture in pancreatic ductal adenocarcinomas Nat Biotechnol … safe dose of lovenox
Sci-Hub Integrating microarray-based spatial transcriptomics and ...
Nettet23. sep. 2024 · Background. The tumor microenvironment (TME) serves as an important factor in tumorigenesis and metastasis. Although distinct cell subsets can be identified … To integrate the scRNA-seq and ST datasets, we developed MIA. This analysis proceeds by first delineating sets of cell type-specific and tissue region-specific genes and then determining whether their overlap is higher (enrichment) or lower (depletion) than expected by chance. In the scRNA-seq data, we … Se mer We first processed fresh primary PDAC tumors from two untreated patients—PDAC-A and PDAC-B—for parallel scRNA-seq and ST analysis (Fig. 1a and see Methods). The scRNA-seq data consisted of cells … Se mer To generate unbiased transcriptomic maps of the tissue sections, we mounted cryosections of the two unfixed PDAC tissues originating from the same tumor sample onto the spatially barcoded ST microarray slides (see … Se mer One of the most useful aspects of scRNA-seq is its ability to reveal distinct subpopulations within cell types. We thus sought to characterize … Se mer We found two cancer cell populations in the PDAC-A scRNA-seq data that appeared to be both genetically and transcriptionally distinct … Se mer NettetAnalyzing seven SRT datasets using SpaGCN, we show it can detect genes with much more enriched spatial expression patterns than competing methods. Furthermore, … ishka trading insolvent