WebPE-Cyanine7 dye. Fluorescence SpectraViewer. PE-Cyanine7 is a tandem fluorophore that can be excited by a broad range of lasers including 488 nm, 532 nm, and 561 nm. Dye … WebPair 1: PE-Cy7 and APC-Cy7 Cy7 portion of PE-Cy7 tandem is responsible for the expected cross-beam emission spilling into the main emission range of APC-Cy7 (red laser excitation). APC-Cy7 fluorescence emission is not expected to significantly spill into the main emission area of PE-Cy7 100(blue laser excitation): 0 10 20 40 50 PE-Cy7 APC …
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WebPE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher. WebNational Center for Biotechnology Information mlb 2019 champions
Compensation of APC-Cy7 and PE-Cy7 - ResearchGate
WebPE-Cy™7 (Ex max 496 nm/Em max 785 nm)is a tandem fluorochrome that combines PE and the cyanine dye Cy7. PE-Cy7 is sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in spillover. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. WebCyanine 7 dyes are popular fluorescent dyes compatible with various detection techniques and standard filters. This class includes near-infrared (IR) fluorescence-emitting dyes with excitation/emission maximum 750/773 nm. 858-677-6760 [email protected] Login Cart Home Products Categories Applications PEG Linkers ADC Linkers WebReagent Selection Guide for the Attune Flow Cytometers. Learning & Support. The Invitrogen Attune cytometers can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry, to match the panels you are currently running. Multiple fluorescent proteins can be interrogated with the 4-laser … mlb 2018 season opener